Publication: Inhibition of chemiluminescence and chemotactic activity of phagocytes in vitro by the extracts of selected medicinal plants
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Date
2010
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Abstract
The methanol extracts of 20 selected medicinal plants were investigated for their effects on the respiratory burst of human whole blood, isolated human polymorpho nuclear leukocytes (PMNs) and isolated mice macrophages
using a luminol/lucigenin-based chemiluminescence assay. We also tested the effect of the extracts on chemotactic migration of PMNs using the Boyden chamber technique. The extracts of Curcuma domestica L., Phyllanthus amarus
Schum & Thonn and C. xanthorrhiza Roxb. were the samples producing the strongest oxidative burst of PMNs with luminol-based chemiluminescence, with IC50 values ranging from 0.5 to 0.7 lg/ml. For macrophage cells, the
extracts which showed strong suppressive activity for luminol-based chemiluminescence were C. xanthorrhiza and Garcinia mangostana L. Among the extracts studied, C. mangga Valton & Vazsjip, Piper nigrum L. and Labisia
pumila var. alata showed strong inhibitory activity on lucigenin-amplified oxidative burst of PMNs, with IC50 values ranging from 0.9 to 1.5 lg/ml. The extracts of Zingiber officinale Rosc., Alpinia galangal (L.) Willd and
Averrhoa bilimbi Linn showed strong inhibition on the chemotaxic migration of cells, with IC50 values compara ble to that of ibuprofen (1.5 lg/ml). The results suggest that some of these plants were able to modulate the innate
immune response of phagocytes at different steps, emphasizing their potential as a source of new immuno-modulatory agents
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Keywords
Medicinal plants Immunomodulatory effect Polymorphonuclear leukocytes Macrophage cells Chemiluminescence Chemotactic activity