Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia

Nor-Aziyah Mat-Rahim; Tengku Rogayah Tengku Abdul Rashid; Jeyanthi Suppiah; Ravindran Thayan; Apandi Mohd Yusof; Zainah Sa'at

doi:https://doi.org/10.1016/s2222-1808(15)60833-7

Publication:
Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia

dc.contributor.author	Nor-Aziyah Mat-Rahim
dc.contributor.author	Tengku Rogayah Tengku Abdul Rashid
dc.contributor.author	Jeyanthi Suppiah
dc.contributor.author	Ravindran Thayan
dc.contributor.author	Apandi Mohd Yusof
dc.contributor.author	Zainah Sa'at
dc.date.accessioned	2024-12-12T14:40:11Z
dc.date.available	2024-12-12T14:40:11Z
dc.date.issued	2015
dc.description.abstract	Objective: To describe the complete nucleocapsid (N) gene region of Middle East respiratory syndrome coronavirus (MERS-CoV) from imported case in Malaysia and the relations with human- and camel-derived MERS-CoV. Methods: Combination of throat and nasal swab specimens was subjected to viral RNA extraction. For screening, the extracted RNA was subjected to real-time RT-PCR targeting upstream of E gene, open reading frame 1b and open reading frame 1a. For confirmation, the RNA was subjected to RT-PCR targeting partial part of the RNA-dependent RNA polymerase and nucleocapsid, followed by amplification of complete N gene region. Nucleotide sequencing of the first Malaysian case of MERS-CoV was performed following the confirmation with real-time RT-PCR detection. Results: Initial analysis of partial RNA-dependent RNA polymerase and N gene revealed that the nucleotides had high similarity to Jeddah_1_2013 strain. Analysis of complete N gene region (1 242 nucleotides) from the case showed high similarity and yet distinct to the nucleotide sequences of camel-derived MERS-CoV. Conclusions: From the finding, there are possibilities that the patient acquired the infection from zoonotic transmission from dromedary camels.
dc.identifier.doi	https://doi.org/10.1016/s2222-1808(15)60833-7
dc.identifier.uri	https://repository.nih.gov.my/handle/123456789/1185
dc.publisher	Asian Pacific Journal of Tropical Disease
dc.relation.ispartof	Asian Pacific Journal of Tropical Disease
dc.relation.issn	2222-1808
dc.subject	Dromedary camels
dc.subject	Imported case
dc.subject	Malaysia
dc.subject	Middle East respiratory syndrome coronavirus
dc.subject	Nucleocapsid gene
dc.title	Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia
dc.type	journal-article
dspace.entity.type	Publication
oaire.citation.issue	7
oaire.citation.volume	5