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Diagnostic Accuracy of Two Dengue NS1 Tests: New Biosensors-Based Rapid Diagnostic Test Versus Enzyme-linked Immunosorbent Assay

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dc.contributor.authorZhuo Lin Chong
dc.contributor.authorShamala Devi Sekaran
dc.contributor.authorHui Jen Soe
dc.contributor.authorDevi Peramalah
dc.contributor.authorSanjay Rampal Lekhraj Rampal
dc.contributor.authorChiu Wan Ng
dc.date.accessioned2024-07-26T04:39:43Z
dc.date.available2024-07-26T04:39:43Z
dc.date.issued2020
dc.description.abstractDiverse clinical manifestation makes early dengue diagnosis difficult. Detection of dengue non structural antigen-1 (NS1) can confirm dengue diagnosis early. This study aimed to compare the diagnostic accuracy of a new biosensors-based rapid diagnostic test (RDT) and an enzyme-linked immunosorbent assay (ELISA) for the detection of dengue NS1 antigen. 91 archived serum samples previously collected from hospitalised patients with suspected dengue were used. 50 cases and 41 controls were ascertained using reverse transcription-polymerase chain reaction, Pan-E Dengue Early ELISA, Immunoglobulin M ELISA, and haemagglutination inhibition. The samples were tested on ViroTrack Dengue Acute and SD Dengue NS1 Ag ELISA by two independent researchers blinded to the reference standard. Statistical analysis was performed using STATA version 12. The sensitivity and specificity of ViroTrack were 92.0% (95%CI 80.8-97.8) and 95.1% (95%CI 83.5-99.4), as compared to 82.0% (95%CI 68.6-91.4) (p=0.03) and 92.7% (95%CI 80.1-98.5) (p=0.32) for the ELISA, respectively. The positive and negative predictive values were 95.8% (95%CI 85.7-99.5) and 90.7% (95%CI 77.9-97.4) for ViroTrack, versus 93.2% (95%CI 81.3-98.6) (p-0.58) and 80.9% (95%CI 66.7-90.9) (p=0.18) for the ELISA, respectively. The diagnostic accuracy of ViroTrack was comparable to ELISA. It may be a more efficient tool for the diagnosis of acute dengue in low resource settings
dc.identifier.doihttps://www.akademisains.gov.my/asmsj/article/diagnostic-accuracy-of-two-dengue-ns1-tests-new-biosensors-based/
dc.identifier.urihttps://repository.nih.gov.my/handle/123456789/718
dc.language.isoen
dc.subjectDengue
dc.subjectEarly diagnosis
dc.subjectDiagnostic accuracy
dc.subjectBiosensors
dc.subjectRapid diagnostic test
dc.titleDiagnostic Accuracy of Two Dengue NS1 Tests: New Biosensors-Based Rapid Diagnostic Test Versus Enzyme-linked Immunosorbent Assay
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