Publication:
A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2

Simple item page
dc.contributor.authorYee Ling Lau
dc.contributor.authorIlyiana Binti Ismail
dc.contributor.authorNur Izati Binti Mustapa
dc.contributor.authorMeng Yee Lai
dc.contributor.authorTuan Suhaila Tuan Soh
dc.contributor.authorAfifah Haji Hassan
dc.contributor.authorKalaiarasu M. Peariasamy
dc.contributor.authorYee Leng Lee
dc.contributor.authorPik Pin Goh
dc.date.accessioned2024-08-22T04:01:59Z
dc.date.available2024-08-22T04:01:59Z
dc.date.issued2020
dc.description.abstractA simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation performed using 50 positive and 32 negative clinical samples showed 100% sensitivity and specificity. The RT-LAMP would be valuable for clinical diagnosis and epidemiological surveillance of SARS-CoV-2 infection in resource-limited areas as it does not require the use of sophisticated and costly equipment.
dc.identifier.doi10.4269/ajtmh.20-1079
dc.identifier.urihttps://www.ajtmh.org/view/journals/tpmd/103/6/article-p2350.xml
dc.identifier.urihttps://repository.nih.gov.my/handle/123456789/996
dc.language.isoen
dc.publisherThe American Society of Tropical Medicine and Hygiene
dc.relation.ispartofThe American Journal of Tropical Medicine and Hygiene
dc.relation.issn0002-9637
dc.relation.journalThe American Journal of Tropical Medicine and Hygiene
dc.titleA Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2
dc.typejournal-article
dspace.entity.typePublication
oaire.citation.endPage2352
oaire.citation.issue6
oaire.citation.startPage2350
oaire.citation.volume103
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
NIH20200230.pdf
Size:
149.29 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Selected Article of Journal (ICR)